Journal: Biomaterials advances

Article Title: The shape of our gut: Dissecting its impact on drug absorption in a 3D bioprinted intestinal model.

doi: 10.1016/j.bioadv.2023.213564

Figure Lengend Snippet: Fig. 3. Fibronectin (gray), vimentin (green), alpha-SMA (green) and collagen type IV (magenta) secretion by HIFs embedded in hydrogels (2.5 × 106 cells/mL) after 21 days in culture. Merged (left) and single channels (right). Fibroblasts presented an elongated shape and were able to produce and secrete ECM proteins into the hydrogel. Pictures depict a maximum intensity projection (z range 250–300 μm) (Scale bar = 50 μm).

Article Snippet: Mouse anti-human vimentin (1:50) (sc-6260, Santa Cruz Biotechnolgy) + rabbit antihuman fibronectin (1:100) (F3648, Sigma) or mouse anti-human alpha smooth muscle actin (alpha-SMA) (1:200) (ab7817, Abcam) + goat antihuman collagen type IV (1:250) (134001, Biorad) were diluted in the primary working buffer (PWB) containing 0.1 % BSA, 0.3 % donkey serum and 0.2 % Triton X-100 in PBS and incubated with the samples overnight (ON), at 4 ◦C in shaking conditions.

Techniques:

Journal: Biomaterials advances

Article Title: The shape of our gut: Dissecting its impact on drug absorption in a 3D bioprinted intestinal model.

doi: 10.1016/j.bioadv.2023.213564

Figure Lengend Snippet: Fig. 7. Protein expression of (a) β-catenin (red), (b) E-cadherin (gray) and fibronectin (yellow), (c) collagen type IV (magenta) and fibronectin (yellow) and (d) β-catenin (red) and ZO-1 (green) in the villi scaffolds after 21 days in culture. * denote the basolateral side of the epithelium (hydrogel). [(a) Scale bar = 100 μm; (b, c) Scale bar = 50 μm].

Article Snippet: Mouse anti-human vimentin (1:50) (sc-6260, Santa Cruz Biotechnolgy) + rabbit antihuman fibronectin (1:100) (F3648, Sigma) or mouse anti-human alpha smooth muscle actin (alpha-SMA) (1:200) (ab7817, Abcam) + goat antihuman collagen type IV (1:250) (134001, Biorad) were diluted in the primary working buffer (PWB) containing 0.1 % BSA, 0.3 % donkey serum and 0.2 % Triton X-100 in PBS and incubated with the samples overnight (ON), at 4 ◦C in shaking conditions.

Techniques: Expressing

Journal: Molecular Vision

Article Title: Downregulation of LIM kinase 1 suppresses ocular inflammation and fibrosis

doi:

Figure Lengend Snippet: LIMK1 siRNA downregulates LIMK1 protein and suppresses fibronectin deposition in human corneal fibroblasts. A : western blot analysis. Corneal fibroblasts were transfected as indicated for 72 h. Cells were lysed and protein levels were analyzed using LIMK1 and GAPGH antibodies. B-E : Immunofluorescence microscopy using fibronectin antibody and DAPI staining in fibroblast cultures ( B ) untreated; ( C ) treated with 100 nM scrambled control siRNA; ( D ) treated with 50 nM of LIMK1 -targeted siRNA; or ( E ) treated with 100 nM of LIMK1 -targeted siRNA. F : Quantitative analysis of fibronectin secreted to the culture media by western blotting. Samples were untreated or treated with control or LIMK1 -targeted siRNA as indicated. Experiments were performed 3 times yielding similar results. Error bars represent standard deviation. The asterisk indicates a p<0.025 (n=3) compared to scrambled siRNA.

Article Snippet: To carry out immunostaining, corneal fibroblasts were incubated with polyclonal rabbit anti-human fibronectin (1:100; BD Biosciences, Lexington, KY) or monoclonal anti-paxillin (1:100; Millipore, Billercia, MA).

Techniques: Western Blot, Transfection, Immunofluorescence, Microscopy, Staining, Standard Deviation

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Sirius red staining of left atrial tissue for connective tissue (red staining) from patients (A) in sinus rhythm (SR) and (B) with chronic atrial fibrillation (lone CAF). Immunohistological results in left atrial tissue from (left) patients in SR and (right) patients with lone CAF for (C, D) collagen type I, (E, F) collagen type III, and (G, H) fibronectin. Original magnification ×400.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Staining

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, collagen III, and fibronectin expression in atrial tissue of (left columns) patients in SR (left columns) and (right columns) patients with atrial fibrillation. (B) Comparison of all patients in the study either (left columns) with underlying mitral valve disease (MVD) or (right columns) without MVD.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing, Comparison

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, (B) collagen III, and (C) fibronectin expression in left atrial tissue of patients in SR, with lone AF, and with AF with underlying MVD (MVD+AF).

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Quantitative analysis of (A) collagen I, (B) collagen III, and (C) fibronectin expression in human atrial tissue of the SR, paroxysmal atrial fibrillation (PAF), and CAF subgroups of lone AF and MVD+AF.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal: Hepatology (Baltimore, Md.)

Article Title: Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation.

doi: 10.1002/hep.21839

Figure Lengend Snippet: Fig. 1. Comparative fibroblasts and MSCs characterization. Morphological aspect of fibroblasts (A, day 13) and MSCs (B, day 15) in first passage growth culture. Immunofluorescence assay showing stainings of fibroblasts (C-F) and MSCs (G-J) for vimentin (C,G), fibronectin (D, H), ASMA (E,I) and laminin (F,J) (n 3 each). Absence of CD200 expression in fibroblasts (K) as shown by immunocytofluorescence assay whereas this marker was positively detected in human neuroblastoma SH-SY5Y cells (n 3). Nuclei were revealed by DAPI staining. Pictures magnifications are (A-J) 400 and (K,L) 200. (M) Example of representative flow cytometric profile of fibroblasts at first passage. Analyzed epitopes are indicated in the histograms. Bars indicate the fluorescence level of corresponding isotype. (N) Comparative cytometric phenotype of MSCs and human skin fibroblasts (both cells from passages 2 to 4). Fibroblasts are indicated by (-f) suffixes.

Article Snippet: Polyclonal anti-human antibodies were incubated overnight at RT at the following concentrations: 1/3000 rabbit anti-human fibronectin, 1/1000 rabbit anti-human FP and 1/200 guinea pig anti-human CK18 (Progen).

Techniques: Expressing, Marker, Staining

Journal: Hepatology (Baltimore, Md.)

Article Title: Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation.

doi: 10.1002/hep.21839

Figure Lengend Snippet: Fig. 8. In vivo hepatocyte differentiation of human skin fibroblasts. Pictures show immunohistochemical stains for human markers fibronectin, FP, albumin, and CK18. Analysis of livers of transplanted SCID-mice revealed that fibroblasts engrafted as small clusters of cells presenting combined expression of mesenchymal marker fibronectin and liver-specific markers FP, albumin, and CK18. Positive and negative control stains are presented, respectively, on human and mouse livers. Abbreviations: FP, alpha-1-fetoprotein. Pictures were taken at 400 magnification.

Article Snippet: Polyclonal anti-human antibodies were incubated overnight at RT at the following concentrations: 1/3000 rabbit anti-human fibronectin, 1/1000 rabbit anti-human FP and 1/200 guinea pig anti-human CK18 (Progen).

Techniques: In Vivo, Immunohistochemical staining, Expressing, Marker, Negative Control